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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Screening and Characterization of Bacteriocin Producing Lactic Acid Bacteria Isolated from Chicken Intestine) ผู้เขียน: คมแข พิลาสมบัติ, วรวิทย์ วัชชวัลคุ,  ดร.สุนีย์ นิธิสินประเสริฐ, รองศาสตราจารย์ , Adisorn Swetwiwathana, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto, ดร.ธวัชชัย ศักดิ์ภู่อร่าม, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractFive hundred strains of lactic acid bacteria (LAB) were isolated from chicken intestine and screened for their abilities to produce bacteriocins. One strain, K4, produced a bacteriocin with activity against Lactobacillus sakei subsp. sakei JCM 1157T, Leuconostoc mesenteroides subsp. mesenteroides JCM 6124T, Bacillus coagulans JCM 2257T, Enterococcus feacalis JCM 5803T, Listeria innocua ATCC 33090T and Brochotrix campestris NBRC 11547T. The antibacterial activity was inactivated by proteolytic enzymes, indicating that it has proteinaceous structure of a bacteriocin. Its properties were heat-tolerance at 121?C for 15 min and stable at wide pH range of 3-10. The mode of action of this bacteriocin was clearly bactericidal activity against Lb. sakei subsp. sakei JCM 1157T and B. coagulans JCM 2257T whereas it showed bacteriostatic action against Br. campestris NBRC 11547T. In addition, K4 strain was able to grow and produce bacteriocin in MRS containing 4% NaCl and at wide pH range of 3-9. Based on morphological, biochemical characteristics and 16S rDNA nucleotide sequence analysis, K4 strain was identified as Lactobacillus salivarius. |
 ผลงานตีพิมพ์ในวารสารวิชาการProcessing and secretion of non-cognate bacteriocins by EnkT, an ABC transporter from a multiple-bacteriocin producer, Enterococcus faecium NKR-5-3ผู้แต่ง:Hirotoshi Sushida, Miyuki Sakei, Rodney H.Perez, Naoki Ishibashi, Takeshi Zendo, Dr.Pongtep Wilaipun, Associate Professor , Dr.Vichien Leelawatcharamas, Associate Professor , Jiro Nakayama, Kenji Sonomoto, วารสาร: 
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ผลงานตีพิมพ์ในวารสารวิชาการMolecular characterization of the possible regulation of multiple bacteriocin production through a three-component regulatory system in Enterococcus faeciumNKR-5-3ผู้แต่ง:Naoki Ishibashi, Naho Matsumoto, Rodney Honrada Perez, Shun Iwatani, Haruki Sugino, Takeshi Zendo, Dr.Pongtep Wilaipun, Associate Professor, Dr.Vichien Leelawatcharamas, Associate Professor, Jiro Nakayama, Kenji Sonomoto, วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการInfluence of Physical Factors and Various Complex Media on Growth and Bacteriocin Production of Two-synergistic Peptide with Heat Stable Bacteriocin Producer, Enterococcus faecium NKR-5-3, Isolated from Thai Fermented Fishผู้แต่ง:Dr.Pongtep Wilaipun, Associate Professor, Takeshi Zendo, Mrs.Mathana Sangjindavong, Associate Professor, Dr.Sunee Nitisinprasert, Associate Professor, Dr.Vichien Leelawatcharamas, Associate Professor, Jiro Nakayama, Kenji Sonomoto, วารสาร: |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Screening and Identification of Effective Thermotolerant Lactic Acid Bacteria Producing Antimicrobial Activity Against Escherichia coli and Salmonella sp. Resistant to Antib ผู้เขียน:ดร.สุนีย์ นิธิสินประเสริฐ, รองศาสตราจารย์, Veeraphol Nilphai, Phunjai Bunyun, ดร.ประกิต สุขใย, รองศาสตราจารย์ , Katsumi Doi, Kenji Sonomoto สื่อสิ่งพิมพ์:pdf AbstractTwo hundred fifty six isolates of lactic acid bacteria (LAB) displaying antimicrobial activity were isolated from chicken intestine. Twenty effective isolates of LAB show bacteriocin – like activity (BLA) against the target strains resistant to antibiotics (Escherichia coli and Salmonella sp.) to varying degrees. Broad spectrum inhibition specific (SIS) were found from 6 h cultivation while the longer cultivation time of 12 and 24 h appeared either the loss of BLA or the inhibition activity against the different target strain. Only 3 effective thermotolerant isolates, i.e., KUB-AC5, KUB-AC16 and KUB-AC20, exerted broad SIS against all target strains except Salmonella sp. S001. However, BLA belonging to KUB-AC5 could not inhibit growth of E. coli E006 and E010 whereas KUB-AC20 did not inhibit only E. coli E006. Based on biochemical, physiological and molecular systematic method, these isolates were classified as Lactobacillus reuteri. |